pgl3‐basic luciferase expression vector (Promega)
90
Structured Review
Promega
pgl3‐basic luciferase expression vector
Pgl3‐Basic Luciferase Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+luciferase+expression+vector/pgl3+basic/pmc11010005-43-21-25
Average 90 stars, based on 1 article reviews
Pgl3‐Basic Luciferase Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+luciferase+expression+vector/pgl3+basic/pmc11010005-43-21-25
Average 90 stars, based on 1 article reviews
pgl3‐basic luciferase expression vector - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Stroke Causes DNA Methylation at Ncx1 Heart Promoter in the Brain Via DNMT1/MeCP2/REST Epigenetic Complex"
Article Title: Stroke Causes DNA Methylation at Ncx1 Heart Promoter in the Brain Via DNMT1/MeCP2/REST Epigenetic Complex
Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
doi: 10.1161/JAHA.123.030460
Figure Legend Snippet: A , Alignment of the human, mouse, and rat Ht sequences is shown. Conserved nucleotides are marked with asterisk. The arrow indicates the TSS. B through E , Luciferase assay in SH‐SY5Y cells transfected for 48 hour with pGL3‐Br ( B and D ) or pGL3‐Ht ( C and E ) and cotransfected with siCTL, siDNMT1, siMeCP2, or siREST ( B , C ), or with EV, DNMT1, MeCP2, or REST ( D and E ). * P ≤0.05 vs siCTL or EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). CTL indicates control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; REST, repressor element 1‐silencing transcription factor; si, small interfering; and TSS, transcription start site.
Techniques Used: Luciferase, Transfection, Plasmid Preparation, Binding Assay, Construct
Figure Legend Snippet: A , Top: JASPAR matrix representation (MA0138.1) of the consensus REST binding site on Ht gene ( Ht ‐RE1). Ht ‐RE1 sequence is represented in International Union of Pure and Applied Chemistry code. Bottom: Partial human genomic Ht sequence containing the predicted REST binding site (underlined). B , Luciferase assay in SH‐SY5Y cells under the following experimental conditions: (1) pGL3basic, (2) pGL3‐Ht+EV, (3) pGL3‐Ht+REST, (4) pGL3‐Ht‐RE1mut+EV, (5) pGL3‐Ht‐RE1mut+REST. * P ≤0.05 vs pGL3Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). C , ChIP with anti‐REST antibody followed by qPCR of the promoter region containing the RE1 site on the Ht gene in SH‐SY5Y cells transiently transfected with (1) pGL3‐Ht+EV, (2) pGL3‐Ht+REST, (3) pGL3‐Ht+RE1mut+REST. * P ≤0.05 vs pGL3‐Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). ChIP indicates chromatin immunoprecipitation; CTL, control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; qRT‐PCR, quantitative real time polymerase chain reaction; RE1, repressor element 1; and REST, repressor element 1‐silencing transcription factor.
Techniques Used: Binding Assay, Sequencing, Luciferase, Transfection, Chromatin Immunoprecipitation, Plasmid Preparation, Construct, Quantitative RT-PCR, Real-time Polymerase Chain Reaction
Related Articles
Polymerase Chain Reaction:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Luciferase:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Expressing:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Plasmid Preparation:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Construct:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Generated:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Amplification:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Clone Assay:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Transfection:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Activity Assay:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Sequencing:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the Purification:Article Title: Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Article Snippet: Reporter constructs pNH11 (2971/184), pNH13 (2556/184), and pNH15 (2160/ 184) were constructed by ligating varying lengths of FATP upstream sequence into the NheI and HindIII sites of the Article Title: Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1. Article Snippet: Zhang, Xianghong, and Youhua Liu.. Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1.. Am J Physiol Renal Physiol 284: F1216–F1225, 2003. Article Title: Genetic variation that determines TAPBP expression levels associates with the course of malaria in an HLA allotype-dependent manner Article Snippet: Nucleotides were added via PCR for insertion between XhoI (5′) and HindIII (3′) restriction sites for Gibson assembly with the Article Title: PPARgamma transcriptionally regulates the expression of insulin-degrading enzyme in primary neurons. Article Snippet: Insulin-degrading enzyme (IDE) is a protease that has been demonstrated to play a key role in degrading both Ab and insulin and deficient in IDE function is associated with Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2) pathology.. However, little is known about the cellular and molecular regulation of IDE expression.. Here we show IDE levels are markedly decreased in DM2 patients and positively correlated with the peroxisome proliferator-activated receptor c (PPARc) levels. Article Title: Hypoxia inducible factors regulate filaggrin expression and epidermal barrier function Article Snippet: Fragments of the human FLG promoter were amplified from keratinocyte genomic DNA using specific primers (available on request) and cloned into Article Title: Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription Article Snippet: The G5-TATA, G5-TATA/INR and G5-INR luciferase constructs were generated by inserting a HindIII DNA fragment containing the five Gal4-binding sites and the core promoter from pG5TdT(-41TATA/+33), pG5TdT(-41TATA/Inr+33) and pG5TdT(-41Inr/+33) [ 28 ] into the HindIII site of Article Title: Enhancers of GnRH Transcription Embedded in an Upstream Gene Use Homeodomain Proteins to Specify Hypothalamic Expression Article Snippet: The fragment was cloned into the Kpn I and Xho I sites of the Article Title: Haplotype-dependent Differential Activation of the Human IL-10 Gene Promoter in Macrophages and Trophoblasts: Implications for Placental IL-10 Deficiency and Pregnancy Complications Article Snippet: The fragments containing the three distinct haplotypes were then digested with Nhe1 and Xho1 restriction enzymes and ligated into the |
